Association Between Glutathione S-Transferase (GST) Gene Polymorphisms and Coronary Artery Disease: A Case-Control Study in Bangladesh.
Glutathione-S-transferases (GSTs) play an important role in the detoxification and neutralization of oxidative stress products and xenobiotic compounds. Due to genetic polymorphisms in GST genes, GSTs partially or completely lose enzymatic activity, which may increase susceptibility to cardiovascular diseases. The objective of this study was to investigate hitherto unknown GSTM1 and GSTT1 null genotype frequencies in patients with coronary artery disease (CAD) and their potential association with CAD in the Bangladeshi population.
This study included 85 patients with CAD and 56 healthy control subjects confirmed by angiogram. Clinical parameters including triglyceride (TG), cholesterol, low-density lipoprotein (LDL), and high-density lipoprotein levels were also evaluated. Genotypes of the GSTM1 and GSTT1 genes were identified using polymerase chain reaction followed by 1.5% (W/V) agarose gel electrophoresis with subsequent detection on a UV transilluminator.
There were significant differences in the null genotype distribution of GSTM1 and GSTT1 alleles between controls and patients with CAD. A significant association of the null genotype with CAD susceptibility (p < 0.05) was also observed. The combination of GSTM1 and GSTT1 null genes potentially increases the risk of CAD (odds ratio = 6.208; 95% confidence interval; p < 0.05). TG, LDL, and serum cholesterol levels were higher in patients with CAD (p < 0.05). GSTM1 and GSTT1 null genetic variations may be associated with increased susceptibility to CAD in the Bangladeshi population.
These findings suggest a potential association between GST gene polymorphisms and CAD susceptibility; however, larger studies are required to provide more robust and generalizable evidence in the Bangladeshi population.
This study included 85 patients with CAD and 56 healthy control subjects confirmed by angiogram. Clinical parameters including triglyceride (TG), cholesterol, low-density lipoprotein (LDL), and high-density lipoprotein levels were also evaluated. Genotypes of the GSTM1 and GSTT1 genes were identified using polymerase chain reaction followed by 1.5% (W/V) agarose gel electrophoresis with subsequent detection on a UV transilluminator.
There were significant differences in the null genotype distribution of GSTM1 and GSTT1 alleles between controls and patients with CAD. A significant association of the null genotype with CAD susceptibility (p < 0.05) was also observed. The combination of GSTM1 and GSTT1 null genes potentially increases the risk of CAD (odds ratio = 6.208; 95% confidence interval; p < 0.05). TG, LDL, and serum cholesterol levels were higher in patients with CAD (p < 0.05). GSTM1 and GSTT1 null genetic variations may be associated with increased susceptibility to CAD in the Bangladeshi population.
These findings suggest a potential association between GST gene polymorphisms and CAD susceptibility; however, larger studies are required to provide more robust and generalizable evidence in the Bangladeshi population.
Authors
Dina Dina, Moni Moni, Emon Emon, Das Das, Kabir Kabir, Muhit Muhit, Sharmin Sharmin, Raihan Raihan
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