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This report describes the excisional biopsy of a lower lip mucocele using a dual-wavelength super-pulsed diode laser (810/980 nm). The procedure was performed under local anesthesia using a 400 μm fiber tip with peak power of 100 W, pulse frequency of 20 Hz, pulse width of 0.05 ms, and average power of 1.2 W. The super-pulsed emission enabled precise tissue incision, effective intraoperative hemostasis, and clear visualization of the surgical field. The excised specimen showed preserved morphology without carbonization, allowing histopathological confirmation of mucocele. After excision, photobiomodulation therapy was applied using a 7-mm adapter (0.3 W, 2 J, 7 s per point, 5.5 J/cm²) to promote postoperative analgesia and tissue repair. Healing was uneventful, with no postoperative pain, edema, or recurrence at 90 days. The dual-wavelength super-pulsed diode laser represents a minimally invasive approach for oral mucocele excision while preserving tissue integrity for histopathological analysis.