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Objective:To establish a standardized operational procedure for T-tube insertion in the treatment of laryngotracheal stenosis and explore its clinical application outcomes. Methods:We established a standardized operational procedure for T-tube insertion treatment in laryngotracheal stenosis, which includes preoperative endoscopic and radiological evaluation, intraoperative endoscopic examination and measurement, airway reconstruction, determination of the length of the superior and inferior limbs of the T-tube, insertion method, as well as postoperative management and efficacy evaluation. This protocol was applied in the treatment of 25 cases of laryngotracheal stenosis to analyze its therapeutic efficacy and safety. Results:The cohort included 25 patients aged 21-72 years（mean age 26±9 years） with a disease duration of 1-30 months（median duration 5 months）. There were 21 males and 4 females. According to the classification method proposed by the European Laryngological Society, 14 cases were classified as Grade Ⅳa, 2 cases as Grade Ⅳb, and 9 as Grade Ⅲa. The T-tube superior limb crossed the glottis in 6 cases and did not cross the glottis in 19 cases. Postoperative follow-up ranged from 12 to 32 months（median 22 months）. A total of 21 patients successfully underwent T-tube removal after the first treatment, yielding a tube removal rate of 84%, among which, 16 patients（64%） were cured, 5 patients（20%） had an effective outcome. In the 4 ineffective cases, 2 patients were cured by secondary sleeve resection and end-to-end anastomosis, while the other 2 patients underwent repeat T-tube placement, and they currently maintain healthy survival with the T-tube blocked, and the long-term efficacy remains to be further observed. Conclusion:T-tube insertion is an effective therapeutic modality for the management of laryngotracheal stenosis. The establishment of a standardized operational procedure and its appropriate application are important guarantees for improving the success rate of treatment.