Exploring hsa_circ_0100833 as a Potential Biomarker in Oral Squamous Cell Carcinoma: Bioinformatics and Experimental Insights.
Oral squamous cell carcinoma (OSCC) is an aggressive malignancy with few reliable early diagnostic markers. Bioinformatic screening identified hsa_circ_0100833 as a differentially expressed circRNA in OSCC. Its potential significance in OSCC was then examined experimentally.
Differential circRNA expression was profiled using the GSE145608 dataset and Limma software. ROC curve analysis, circRNA-miRNA-mRNA interaction prediction, functional enrichment, and survival analysis were then used. Hsa_circ_0100833 expression was measured by qRT-PCR in 20 paired OSCC and adjacent normal tissues. This analysis was also performed in CAL-27 and FaDu cell lines (n = 3 each) and three normal human gingival fibroblast cultures. hsa-miR-607 and SORBS1 were quantified in parallel.
Differential expression analysis of the GSE145608 dataset identified hsa_circ_0100833 as down-regulated in OSCC. Bioinformatic analysis then predicted a binding interaction between this circRNA and hsa-miR-607. Hsa_circ_0100833 was significantly reduced in OSCC tissues and cell lines relative to normal controls; hsa-miR-607 was up-regulated, and SORBS1 showed parallel downregulation. ROC analysis revealed AUC values of 0.799 in cell lines and 0.712 in tissue samples. The predicted ceRNA network was enriched for PI3K-Akt signaling and focal adhesion pathways.
hsa_circ_0100833 was down-regulated in OSCC and may act as a tumor suppressor through a hsa-miR-607/SORBS1-dependent mechanism. Its diagnostic potential warrants further investigation.
Differential circRNA expression was profiled using the GSE145608 dataset and Limma software. ROC curve analysis, circRNA-miRNA-mRNA interaction prediction, functional enrichment, and survival analysis were then used. Hsa_circ_0100833 expression was measured by qRT-PCR in 20 paired OSCC and adjacent normal tissues. This analysis was also performed in CAL-27 and FaDu cell lines (n = 3 each) and three normal human gingival fibroblast cultures. hsa-miR-607 and SORBS1 were quantified in parallel.
Differential expression analysis of the GSE145608 dataset identified hsa_circ_0100833 as down-regulated in OSCC. Bioinformatic analysis then predicted a binding interaction between this circRNA and hsa-miR-607. Hsa_circ_0100833 was significantly reduced in OSCC tissues and cell lines relative to normal controls; hsa-miR-607 was up-regulated, and SORBS1 showed parallel downregulation. ROC analysis revealed AUC values of 0.799 in cell lines and 0.712 in tissue samples. The predicted ceRNA network was enriched for PI3K-Akt signaling and focal adhesion pathways.
hsa_circ_0100833 was down-regulated in OSCC and may act as a tumor suppressor through a hsa-miR-607/SORBS1-dependent mechanism. Its diagnostic potential warrants further investigation.
Authors
Raei Raei, Seyedena Seyedena, Hashemi Hashemi, Hosseinkhan Hosseinkhan, Alaeddini Alaeddini
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