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Declining testosterone levels are associated with reduced muscle mass, fatigue, and reproductive challenges in men. Although testosterone replacement therapy is available, it is often associated with risks such as cardiovascular complications and endocrine imbalance, highlighting the need for safer alternatives. Nutraceutical approaches offer a promising strategy for supporting endogenous testosterone production. In this study, Testolift, a novel testosterone-supporting nutraceutical formulation, was developed under the Natural Ingredients for Mental and Performance (NIMP) Platform using InSitu360 molecular complexation technology. The formulation integrates fenugreek saponins, black ginger (Kaempferia parviflora) polymethoxyflavones, and zinc methionine to achieve synergistic enhancement of testosterone. The formulation was characterized using FT-IR, SEM, TEM, DSC, EDS, and UPLC, confirming the successful integration of bioactives and favorable physicochemical properties. Quantitative analysis verified the presence of 20% saponins, 0.93% polymethoxyflavones, and 1.4% elemental zinc. Morphological studies revealed uniformly dispersed spherical nano- and microspheres with sub-200 nm particles, supporting structural stability and efficient incorporation of bioactives within the matrix. Dynamic light scattering analysis confirmed a mean particle size of 193 nm with a distribution range of 120-260 nm, while the zeta potential of -32.24 mV indicated good colloidal stability. A 180 days stability study demonstrated >95% retention of methoxyflavones, saponins, and zinc across refrigerated (4 ± 2 °C), ambient (25 ± 2 °C), and accelerated (45 ± 2 °C) conditions, confirming excellent compositional and physicochemical stability. In a 42 days in vivo experimental study in male rats, Testolift significantly increased serum testosterone by 22.7% compared to 4.1% in controls (p < 0.05) without adverse effects. The InSitu360 technology enhanced the physicochemical and compositional stability of the formulation, supporting its robustness and suggesting potential for improved bioavailability, which warrants confirmation in future clinical studies. These results position Testolift as a promising natural testosterone-support formulation with potential applications in male health and muscle endurance.