Feed

Immune cell intravasation into the tumor is an absolute requirement for the immune system to exert control on tumor growth. Its regulation directly influences immune surveillance and therapeutic response to immunotherapies and other immune cell-dependent cancer therapies. Despite its significance, investigation of this process remains a major challenge due to technical limitations. Intravital microscopy (IVM) has emerged as an essential tool for visualizing dynamic cellular interactions within the tumor microenvironment. This study presents an optimized IVM-based technique to track immune cell interactions with tumor endothelium in a preclinical model. Our approach combines high-resolution imaging with fluorescent labeling strategies to capture real-time interactions between immune and endothelial cells. By providing an enhanced method for studying immune infiltration in tumors, this technique could contribute to a better understanding of the tumor microenvironment and the development of novel therapeutic strategies.