Non-invasive pulsed ultrasound enhances hematoma clearance and neurological recovery in experimental intracerebral hemorrhage.
Intracerebral Hemorrhage (ICH) is a devastating stroke subtype. Accelerating hematoma clearance is a critical therapeutic goal. This study evaluated non-invasive pulsed ultrasound for enhancing hematoma clearance, edema resolution, and recovery post-ICH.
Twenty-nine rats with striatal autologous blood-induced ICH were randomized into control (n = 11), 2 MHz ultrasound (n = 7), and 8 MHz ultrasound (n = 11) groups. Ultrasound treatment (60 min/day) was applied for 7 consecutive days following ICH induction. Hematoma volume and perihematomal edema (PHE) were assessed by T2-weighted imaging (T2WI) and susceptibility-weighted imaging (SWI) at days 1 and 7 post-ICH. Neurological function was assessed by corner turn and cylinder tests at baseline and days 1, 3, and 7.
Non-invasive pulsed ultrasound significantly enhanced hematoma clearance (2 MHz: 47.7%; 8 MHz: 47.8% vs. control: 20.4%, p < 0.01) and PHE resolution (2 MHz: 53.9%; 8 MHz: 71.8% vs. control: 31.1%, p < 0.05). Behavioral tests showed reduced right-turn bias and forelimb asymmetry in ultrasound groups (p < 0.05). No frequency difference was found.
Non-invasive pulsed ultrasound significantly enhances hematoma clearance, reduces edema, and improves functional recovery post-ICH, supporting its translational potential.
Twenty-nine rats with striatal autologous blood-induced ICH were randomized into control (n = 11), 2 MHz ultrasound (n = 7), and 8 MHz ultrasound (n = 11) groups. Ultrasound treatment (60 min/day) was applied for 7 consecutive days following ICH induction. Hematoma volume and perihematomal edema (PHE) were assessed by T2-weighted imaging (T2WI) and susceptibility-weighted imaging (SWI) at days 1 and 7 post-ICH. Neurological function was assessed by corner turn and cylinder tests at baseline and days 1, 3, and 7.
Non-invasive pulsed ultrasound significantly enhanced hematoma clearance (2 MHz: 47.7%; 8 MHz: 47.8% vs. control: 20.4%, p < 0.01) and PHE resolution (2 MHz: 53.9%; 8 MHz: 71.8% vs. control: 31.1%, p < 0.05). Behavioral tests showed reduced right-turn bias and forelimb asymmetry in ultrasound groups (p < 0.05). No frequency difference was found.
Non-invasive pulsed ultrasound significantly enhances hematoma clearance, reduces edema, and improves functional recovery post-ICH, supporting its translational potential.
Authors
Ma Ma, Wang Wang, Wang Wang, Du Du, Dong Dong, Li Li, Guan Guan, Xing Xing, Gong Gong, Dong Dong, Guo Guo, Ji Ji
View on Pubmed