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The increasing accessibility of next-generation sequencing has empowered researchers to investigate somatic mutations in cancer. The complexity of variant analysis pipelines, terminology, and tool selection remains a major barrier, especially for those new to the field or working in translational settings. To address this challenge, we present a practical framework that guides researchers through the critical steps of variant interrogation in tumor samples. This guide is broken into four phases: Planning-laying the foundation for thoughtful experimental design and a clear understanding of sequencing outputs; Gathering Resources-assembling the tools, reference data, and variant annotation sets required for analysis; Filtering and Validation-executing a systematic approach to prioritize meaningful variants; and Dissemination and Storage-ensuring findings are reproducible and accessible through transparent reporting and data sharing. Developed with an emphasis on accessibility, reproducibility, and clinical relevance, this framework equips researchers with the guidance to navigate variant analysis with confidence and rigor.