Uncontrolled hypertension can result from untreated high blood pressure (BP) or the inefficacy of established antihypertensive therapeutic regimens. Renal denervation (RDN) is a nonpharmacologic catheter-based intervention that achieves targeted renal sympathetic nerve ablation to modulate sympathetic activation. RDN is suitable for those with uncontrolled primary hypertension, resistant to therapy or intolerant to drugs, and who have a favorable renal artery anatomy. Long-term data demonstrate RDN's efficacy in significantly reducing elevated BP. RDN procedures have shown a good safety profile, and no significant difference in adverse events has been reported between RDN-treated and control groups in most clinical trials. Thus, RDN offers an effective and safe approach for sustained BP control.

Salvia miltiorrhiza(SM)-Dalbergia odorifera (DO) is a commonly used circulating blood and transforming stasis drug pair for the treatment of cardiovascular and cerebrovascular diseases, but systematic studies on the optimal ratio for the treatment of cerebrovascular diseases have not been reported. In this study, we determined the optimal ratio of SM-DO for the treatment of ischemic stroke (IS) through the mixture design.Five different ratios of SM and DO were applied to middle cerebral artery occlusion (MCAO) mice using an enhanced simplex center-of-mass mixture design by Minitab 17 software. The experimental ratios of SM and DO as independent variables and dependent variables including modified neurological severity scores, corner test, sticker removal test, cylinder test, 2,3,5-triphenyltetrazolium chloride staining, lactate dehydrogenase (LDH) and neuron-specific enolase (NSE) levels, and the optimal ratio of the two herbs was obtained by fitting regression equations through mixture design and multi-objective synchronization optimization. The therapeutic effects of the optimal ratio of SM-DO on MCAO mice were verified and evaluated by the indicators of neurological function, brain swelling, cerebral infarction volume, brain histopathological morphology, and the levels of LDH and NSE. The optimal ratio of SM:DO = 0.61:0.39 was obtained by multi-objective synchronization optimization. The results of pharmacodynamic validation showed that the optimal ratio of SM-DO significantly improved the neurological function scores of MCAO mice, the motor functions of the left forelimb, the sensory functions of the left paw, the motor functions of the right steering, the cerebral infarct volume, and the release of LDH and NSE and exerted the therapeutic effect on IS. The optimal ratio of SM (0.61) and DO (0.39) was found to be effective in the treatment of IS mice based on mixture design.

BACKGROUND Renal pseudoaneurysms can occur following penetrating renal trauma, renal surgery, or renal biopsy, and are a cause of delayed bleeding. However, cases occurring after blunt trauma are rare, and pseudoaneurysms may become apparent in a delayed fashion after initially negative imaging. CASE REPORT A 52-year-old man sustained trunk injuries in a motorcycle accident, including left renal injury (American Association for the Surgery of Trauma [AAST] Grade III) and splenic injury (AAST Grade IIIa or IIIb). Transcatheter arterial embolization was performed for the splenic injury, and the left kidney was managed conservatively. The patient developed fever on post-injury day 5, and computed tomography on day 6, performed to evaluate the fever source, did not demonstrate a pseudoaneurysm. Gross hematuria later worsened, with progressive anemia. On day 12 after injury, a new pseudoaneurysm was identified, and transcatheter arterial embolization was performed using n-butyl-2-cyanoacrylate and microcoils. Additionally, a ureteral stent was placed due to concomitant urinary fistula. Subsequently, fever and hematuria improved, and the patient was transferred to the urology ward on day 20 after injury. Urinary extravasation can contribute to pseudoaneurysm formation; therefore, repeat imaging and timely intervention should be considered in selected high-risk patients. CONCLUSIONS Delayed detection of renal segmental artery pseudoaneurysm should be considered after blunt renal trauma. Minimally invasive endovascular treatment is effective and kidney-sparing. Concomitant urinary extravasation and urinary fistula may contribute to delayed bleeding complications; therefore, repeat imaging and timely intervention should be considered in selected high-risk patients.

The vast majority of individuals with autosomal recessive (AR) conditions demonstrate biparental inheritance of the disease-causing alleles; however, de novo variants also contribute to AR disease. This report represents the largest cohort to-date of rare AR conditions in which one of the disease-causing alleles was inherited and one occurred de novo. Clinical and research staff at Stanford University, clinical sites of the Undiagnosed Diseases Network (UDN) and Genomics Research to Elucidate the Genetics of Rare diseases (GREGoR) Consortium, and a large clinical genetic testing laboratory were contacted to identify cases of an AR diagnosis resulting from an inherited and de novo disease-causing variant in trans. Fifteen cases of AR conditions caused by one inherited and one de novo variant in a gene consistent with the clinical phenotype were identified; all had undergone trio exome or genome sequencing with genetic confirmation of reported relationships. Variants were confirmed to be in trans in eight of the 15 cases. The de novo variant was confirmed (n = 7) or presumed (n = 7) to have arisen on the paternal allele in 14/15 (93%) of cases. Phenotypic and/or molecular evidence of an AR condition should prompt parental segregation analysis to inform diagnosis, recurrence risks, and variant classification. Additional studies are needed to determine the incidence of this phenomenon given the implications for the interpretation of genetic testing and counseling for AR conditions.

Agrimonia pilosa Ledeb. (APL) is an edible and medicinal plant, which has a favorable cardioprotective effect. Myocardial fibrosis (MF) is a hallmark pathological feature of various cardiovascular diseases. This study aims to evaluate its protective effects against isoproterenol (ISO)-induced MF and investigate the underlying mechanisms. HPLC was employed to analyze the main active ingredients in APL. Network pharmacology methods, combined with experimental validation, elucidated the mechanism by which APL modulates mitophagy to alleviate MF. HPLC analysis showed that six ingredients were identified. We demonstrated that APL significantly attenuated myocardial injury, enhanced cardiac function, inhibited oxidative stress and apoptosis, and effectively ameliorated MF progression. Network pharmacological predictions and in vivo experimental validation demonstrated that APL exerts its therapeutic effects through regulation of the FOXO signaling pathway and suppression of excessive mitophagy. Furthermore, we artificially elevated FOXO1 expression in vitro, which reversed the effects of APL, as evidenced by increased expression of mitophagy and fibrosis-related proteins. Consistent results from both in vivo and in vitro experiments demonstrated that APL attenuates ISO-induced MF and suppresses mitophagy mediated by the FOXO signaling pathway.

MicroRNA (miRNA) stands for a class of small, non-coding RNA molecules, typically forming 20 to 25 nucleotides in length, which play a pivotal role in the regulation of gene expression in eukaryotic cells. These molecules are integral to the post-transcriptional regulation of target messenger RNA (mRNA), which they achieve primarily through binding to complementary sequences within the 3' untranslated region (UTR) of the mRNA. miRNAs exert their regulatory effects by binding to their target mRNA, leading to two primary outcomes: mRNA degradation or inhibition of translation. This functionality positions miRNAs as crucial modulators of a variety of biological processes, including cell growth, differentiation, apoptosis, and responses to environmental stress. Furthermore, dysregulation of miRNA expression is associated with pathological conditions, including cancer, cardiovascular disease, and neurological disorders. They are associated with sleep regulation and are helpful in diagnosing diseases, including sleep disturbances. This scoping review aims to summarize existing literature in patients aged 18 to 65 with a main diagnosis of Short Sleep Disorder (SSD), and Insomnia with Short Sleep Duration (ISOSS) and its relationship with miRNAs. The objective of this study is to search for existing evidence on the composition (presence, diversity, and relative abundance) of miRNAs in individuals with and without sleep disturbances, and to know whether the cardiometabolic comorbidities associated with these pathologies imply miRNA alterations.

This scoping review was performed according to the PRISMA-ScR checklist and the systematic literature search was conducted using Medline, Web of Science, and CINAHL, until June 2025.

Seven cross-sectional studies form the basis of this ScR. The miRNAs of particular interest include miR-182-5p, miR-4433b-3p, which are implicated in inflammatory pathways, and appear to be downregulated in conditions associated with sleep deprivation, thereby contributing to an exacerbation of pro-inflammatory responses that can lead to cardiovascular dysfunction. Also, miR-619-5p, linked to stress responses and cognitive and emotional health, miR-33a, miR-181d, implicated in lipid metabolism and neuroinflammation, miR-132-3p linked to increased risk of depression and cognitive decline and miR-125a, miR-126, and miR-146a, critical regulators of diverse biological processes, particularly in the context of inflammation and cardiovascular health.

The existing literature sets up a foundational understanding of the relationship between miRNAs, insomnia (IS), SSD and ISOSS. Although the current evidence base is limited, dysregulation of specific miRNAs may play a role in the pathophysiology of IS, SSD and ISOSS; however, larger and methodologically standardized studies are required.

E3 ubiquitin ligases play essential roles in catalyzing the ubiquitination process and are involved in almost all life activities of eukaryotes. RNF20 is a really interesting new gene (RING) finger E3 ubiquitin ligase and is well-known for its role in monoubiquitination of histone H2B. Recent studies have offered new insights into how RNF20 regulates gene expression and developmental programs, and how misregulation of its activities leads to pathologies including developmental disorders and cancers. Here, we provide a current review of the physiological and pathological roles of RNF20 in embryonic development and human diseases, and outline its cellular and molecular modes of action, as well as the upstream control of RNF20 activity, thus providing insights for the molecular basis of RNF20 misregulation associated human diseases.

Uncoupling proteins (UCPs) are transmembrane proteins located in the inner membrane of mitochondria. They can reduce the efficiency of ATP synthesis and promote heat production by mediating the uncoupling oxidative phosphorylation process. Different subtypes of UCPs have distinct tissue distributions and functional characteristics, involving various biological processes including temperature regulation, energy balance, signal transduction, oxidative stress, and the inflammatory response. In recent years, many studies have shown the potential value of UCPs in the prevention and treatment of metabolic diseases such as obesity, diabetes, cardiovascular diseases, neurological diseases, and tumors. Importantly, multiple evidence reveals that innovative therapies targeting uncoupling proteins will show broad application prospects in the future. This review of recent research on UCPs aims to provide a direction for exploring the molecular mechanisms of cellular homeostasis and intervention strategies for metabolic diseases.

Expanding psychostimulant prescribing to general practitioners (GPs) addresses Australia's attention deficit hyperactivity disorder (ADHD) access crisis, but risks significant unintended consequences. This article highlights the cumulative risks of long-term stimulant use and the danger of fragmented care models. We argue that inconsistent training and the rise of vertically integrated telehealth platforms threaten to reduce ADHD care to a volume-based pharmacological service. To protect public health, Australia requires a national framework prioritising mandatory education, shared care pathways and periodic reviews. Success must be measured by the quality of holistic care, not merely script volume.

The study aimed to examine the relationship between lifestyle, night shift pattern, mental health and nurses' metabolic diseases.

We included 910 nurses from 2018 to 2022. The Growth mixture model was used to identify the trajectories of metabolic diseases among nurses. And multinomial logistic regression was used to examine the relationship between lifestyle, night shift pattern, mental health and the trajectories of metabolic diseases.

Three distinct trajectories were identified: Maintaining-Low, Chronically-High group, Maintaining-Low group. Compared to the Maintaining-low group, the correlates of Chronically-high group were lack of dietary preference for vegetables and exercise. Low depression scores, high anxiety scores, night shift pattern with a slow increase in metabolic diseases.

The changes of the number of metabolic diseases among nurses in China are heterogeneous. Lack of dietary preference for vegetables and exercise are significantly related to nurses' metabolic disorders. Among nurses with high initial health level, the correlates of the increase in the number of metabolic diseases are not unhealthy lifestyles, but mental health and night shift pattern.