Brief, accurate, and context-sensitive screening tools are essential for detecting gambling-related problems, but most existing instruments are not validated for on-site gambling.

This study aimed to estimate the prevalence and patterns of Gambling Disorder (GD) and problem gambling among Brazilian lottery players and to develop and test a concise screening tool optimized for this on-site context.

A multistage, stratified sampling design recruited 5,407 adult gamblers at 494 lottery kiosks. Participants completed a version of the NODS adapted to DSM-5 criteria. Using an exhaustive combinatorial approach, all 2-4-item subsets from the NODS were tested for sensitivity and specificity.

Three combinations emerged as optimal. A three-item version (control, coping, chasing) showed the best balance for detecting problematic gambling (sensitivity = 96.5%), while a four-item version reached high specificity for GD (96.2%). All combinations outperformed the widely used NODS-CLiP.

The study introduces the "3-Cs screener," a novel, ultra-brief tool for on-site lottery players. Its high diagnostic accuracy makes it a promising candidate for prevention and intervention strategies, though further validation in clinical and digital settings is warranted.

Open Dialogue (OD) is a social network-based, integrative approach to mental health care that encourages shared understanding between service users, their significant others, and professionals.

This study aimed to explore staff experience of OD training delivered across services in planned, acute and urgent care, providing a multi-team approach.

Focus groups were conducted with seventeen staff members across two time points: three months into training, and three months post-training. A thematic analysis was performed on the transcribed data.

Four superordinate themes, each containing multiple subthemes, were identified across the data set: current practice versus paradigm shift, commitment of training, friction in experiential learning, and shifting power.

Trainees offered mixed evaluations of the training in relation to its practical and experiential elements, with considerable value being found in the multi-team approach. The findings suggest a range of clinical, organisational, and personal benefits of OD training for members of staff through; renegotiating power dynamics, improving working relationships and continuity of care, and enhancing personal reflection and development.

The menopause transition (MT) can present with a range of biopsychosocial challenges, including peaks in anxiety and depression. Compassionate Mind Training (CMT), a psychological intervention focused on improving distress and wellbeing, has shown promising results in supporting depression in clinical populations. This study examined the feasibility and preliminary effects of a six-session online CMT group intervention for women in MT, with mild to moderate anxiety and/or mild to moderately severe depression.

This was a mixed-methods feasibility study. 38 participants were randomized to either CMT plus treatment as usual (TAU) or TAU alone. Feasibility and acceptability outcomes included recruitment, retention, attendance, and measure completion. Exploratory analyses examined changes in psychological outcomes. Qualitative interviews (n=16) were analyzed thematically to explore participant experiences.

Recruitment targets were met, with retention rates of 89.5% for CMT, and 73.7% for TAU. Attendance was high, and outcome measures showed 100% completion among those at follow-up. Exploratory analyses suggested improvements following CMT for depression (Patient Health Questionnaire-9, d=0.73), anxiety (Generalized Anxiety Disorder-7, d=0.53), and anxiety/depressive symptoms (Women's Health Questionnaire, d=1.12), alongside reductions in fears of compassion and self-criticism. Thematic analysis generated five themes: motivation and engagement, perceptions of trial processes, structure, support, and group experience, intervention content and skill-building, and personal outcomes, impact, and the future.

The study supports the feasibility and acceptability of CMT for women in MT. Quantitative and qualitative findings suggest potential benefits for mental health and quality of life, warranting a fully powered trial of effectiveness and cost-effectiveness.

Chronic atrial fibrillation (CAF) is the most frequent sustained cardiac arrhythmia, and it is associated with a high burden of symptoms that impair self-care behaviors, treatment adherence, and quality of life.

In this study, our aim was to estimate the prevalence of anxiety and depressive symptoms in individuals with CAF and identify associated factors.

A cross-sectional analytical study was conducted at the cardiology outpatient clinic of a university hospital in João Pessoa (Paraíba, Brazil). Anxiety and depressive symptoms were assessed using the Hospital Anxiety and Depression Scale. Data were analyzed using the Chi-square test, prevalence ratio with a 95% confidence interval, and Poisson regression with robust variance.

The study included 102 individuals with CAF; 60.8% reported anxiety symptoms and 38.2% reported depressive symptoms. The prevalence of anxiety symptoms was significantly lower in men (39.0%) than in women, and 27.0% lower among individuals with 8 years of education or less than in those with 9 years or more. The prevalence of depressive symptoms was 59.0% lower in men than in women, and 43.0% lower in individuals with up to 3 comorbidities than in those with 4 or more comorbidities.

Among individuals with CAF, factors associated with anxiety and depressive symptoms were identified, including sex, educational level, and the number of comorbidities. These findings underscore the importance of incorporating systematic mental health assessment and integrative care strategies into the nursing management of individuals with CAF.

Extensive literature has documented the psychological burden of chronic kidney disease and kidney failure. Psychological resilience, the ability to adapt to challenging life experiences, offers a critical lens for understanding the variability in how individuals experience and adapt to chronic medical conditions. We aimed to synthesize existing evidence that has explored resilience among patients with kidney diseases.

This scoping review followed the JBI Scoping Review framework. Empirical studies that measured resilience with no time restriction were considered and resilience could be an exposure, outcome, or variable.

Of the 4525 reports screened, 36 studies (33 observational, 3 interventional) from 11 countries that included 6028 patients met the inclusion criteria. Most studies focused on patients on hemodialysis (n = 30). Multiple studies reported that resilience was negatively correlated/associated with depression (n = 10), and anxiety (n = 7), as well as psychological vulnerabilities. A positive correlation between resilience and post-traumatic growth, coping, and mental well-being was reported. Higher resilience positively correlated with measures of physical health (health-related quality of life, frailty, and instrumental activities of daily living). Factors, such as religiosity, spirituality, health-promoting behaviors, social support, family functioning, and family resilience, were enablers of resilience. Three studies reported positive impacts of resilience-enhancing interventions.

Lower resilience is associated with depression and other inferior psychological and physical outcomes in patients with kidney diseases. Psychological resilience is measurable and modifiable. Given the multifaceted and often debilitating nature of kidney diseases and kidney failure, we propose that resilience can help to improve patient outcomes.

Long-term care (LTC) is a fundamental system in many countries that provides essential aged care services and promotes the physical and mental well-being of older adults. This study visually maps the progress and trends in LTC research, offering theoretical references for fellow scholars and practitioners. Using the Web of Science Core Collection as the data source, we retrieved publications on LTC from 2010 to 2024. CiteSpace bibliometric software was employed with a time slice of 1 year and a selection threshold of top 50 per slice. Co-occurrence and collaboration networks were analyzed for keywords, authors, and institutions. The United States contributed the highest number of publications (1896). Among journals, The Lancet published the most articles (2568). The University of Toronto exhibited the strongest centrality (0.23). The top 5 most frequent keywords were "long-term care" (1666 occurrences), "dementia" (726), "nursing home" (674), "health" (615), and "older adult" (551). Qualitative synthesis further identified major research themes including long-term mortality among LTC recipients, nursing home residents, LTC insurance, pragmatic trials, and social isolation. Author collaboration networks showed only small clusters with weak overall connectivity; institutional collaborations were also limited, predominantly involving universities. This fragmented cooperation pattern did not improve significantly over time, providing a critical benchmark for evaluating the effectiveness of academic community building within the field. Notably, all high-frequency keywords exhibited extremely low centrality values (0.01-0.02), indicating that while a large volume of research centers on a few core terms, the knowledge linkages between these topics and broader research issues remain weak. Additionally, burst detection revealed strong emergence of terms such as "unit" and "setting," further highlighting an exceptionally high concentration of research attention on micro-level care facility issues. Future research should strengthen international collaboration at the macro-level, expand the target population of LTC to a wider range of older adults at the meso level, and address the chronic disease needs of older individuals at the micro-level, thereby advancing the development of LTC systems.

Pelvic organ prolapse (POP) significantly impacts women's physical and mental health. Although current research primarily focuses on the analysis of extracellular matrix (ECM) components, the underlying pathological imbalances of POP remain incompletely understood. The functional roles and phenotypic changes of fibroblasts within the complex microenvironment of prolapsed tissue warrant further investigation.

To decipher the pathological basis of pelvic organ prolapse (POP) at single-cell resolution through analyzing the heterogeneity of fibroblasts, exploring prolapse-specific fibroblast subtypes and their functional characteristics.

A total of 6 full-thickness prolapsed vaginal wall tissue samples were collected from patients with stage III or IV pelvic organ prolapse (POP group), along with 3 control tissue samples from patients with stage 0-I prolapse who underwent total hysterectomy for benign gynecological diseases (CTRL group). Nuclei were isolated from frozen tissues and processed into single-nucleus suspensions for snRNA-seq. The sequencing data were subjected to dimensionality reduction, clustering, cell type annotation, and subpopulation identification to construct a single-nucleus transcriptome atlas of the anterior vaginal wall, with a focused exploration of fibroblast heterogeneity. Gene Ontology Biological Process (GO BP) enrichment analysis was performed to investigate dysregulated cellular functions and the functional characteristics of fibroblast subpopulations. Pseudotime trajectory analysis was performed to construct fibroblast differentiation trajectories and investigate fibroblast subpopulation specific differentiation aberrations. The main findings of bioinformatics analysis were validated by immunofluorescence staining and in vitro functional assays.

The transcriptomes of 96,622 vaginal wall cell nuclei isolated from POP and CTRL group were profiled. Fibroblasts, endothelial cells, and epithelial cells were the major cell populations in the anterior vaginal wall. Fibroblasts exhibited significantly higher enrichment scores for extracellular matrix (ECM) and related gene sets compared to other cell types, underscoring their critical role in regulating ECM homeostasis in pelvic floor tissues. In the POP group, we observed an increased proportion of pro-inflammatory (IL6ST⁺_FIB) and matrix-degrading (MMP2⁺_FIB) fibroblast subpopulations, alongside a decreased proportion of ECM-synthesis (COL1A1⁺_FIB) and mesenchymal (POSTN⁺_FIB) fibroblast subpopulations. Pseudotime trajectory analysis further revealed that fibroblast differentiation in POP samples shifted away from an ECM-synthesis phenotype toward pro-inflammatory and matrix-degradating phenotypes. Functional enrichment analysis of fibroblasts showed that biological processes related to ECM remodeling and negative regulation of cell migration and growth were upregulated. Consistently, fibroblasts isolated from prolapsed tissue exhibited impaired proliferation and migration capabilities.

Fibroblasts in prolapsed vaginal wall exhibited marked heterogeneity compared to non-prolapsed tissue, comprising 6 functionally distinct subpopulations, including those involved in ECM synthesis, pro-inflammatory, and matrix degradation. In the prolapse microenvironment, an aberrant phenotypic shift was observed that fibroblasts transitioned from an ECM-stable phenotype predominantly characterized by COL1A1⁺_FIB toward pro-inflammatory (IL6ST⁺_FIB) and matrix-degrading (MMP2⁺_FIB) phenotypes, which contributes to extracellular matrix disorganization and impaired tissue repair capacity. These findings further enhance the understanding of the fibroblast phenotype imbalance underlying POP and provide novel therapeutic strategies targeted at modulating fibroblast function to restore pelvic floor.

While the health impacts of physical activity are well-established, the contribution of internal regulatory processes to these behaviors remains unclear. This study adopts an integrative perspective to examine how interoceptive sensibility and emotion regulation jointly differentiate physical activity levels and predict attitudes toward effort.

1073 adults (557 males, mean age = 35.4) completed the Beck Depression Inventory, State-Trait Anxiety Inventory, Cognitive Emotion Regulation Questionnaire, Multidimensional Assessment of Interoceptive Awareness, and Physical Effort Scale. Participants were classified into three physical activity groups (Inactive, Moderate, High) using the International Physical Activity Questionnaire (IPAQ), based on established metabolic equivalent and frequency criteria. The IPAQ also assessed total sitting time.

ANCOVAs controlling gender, age, education and BMI, showed that Moderate and High groups demonstrated higher interoceptive sensibility than the Inactive group. Inactive group exhibited higher anxiety and depression symptoms and greater attention to uncomfortable sensations. No differences emerged between the Moderate and High groups in emotional regulation or interoception. Approach and Avoidance toward physical effort significantly differed across all three physical activity levels. Multiple regression indicated that low worry about bodily sensations predicted greater Approach and lower Avoidance toward physical effort. Greater listening to body sensations and not distracting from them predicted higher Avoidance, while higher self-regulation by focusing on body sensations predicted higher Approach.

Cognitive emotion regulation did not differentiate physical activity groups, whereas attitudes did and were mainly predicted by interoception, highlighting the role of sensory-perceptual processes. Targeting attitudes toward exercise and interoceptive processes may promote physical activity, with moderate levels may be sufficient for mental health benefits.

Depression is a common psychiatric condition posing serious threats to individual physical and mental health. While previous studies have demonstrated that trait emotional regulation (TER) is a protective factor for depression, the neural correlates between TER and depression remain unclear, especially in adolescents. This study aimed to investigate the neurofunctional substrates of TER and its neural link to depressive symptoms in adolescents.

Resting-state functional magnetic resonance imaging and a battery of behavioral assessments were administered to 231 general high school students. Whole-brain correlation analyses and prediction analyses were conducted to identify brain regions whose fractional amplitude of low-frequency fluctuations (fALFFs) were associated with TER. We examined the correlation between the identified brain regions and depressive symptoms, and conducted mediation analyses to explore the role of TER in mediating the brain-depression relationship.

TER was significantly correlated negatively with fALFF in the ventromedial prefrontal cortex (VMPFC), and positively with fALFF in the cuneus. Depressive symptoms were negatively correlated with TER, and positively with fALFF in the VMPFC. In mediation analysis, TER had a significant indirect effect on the relationship between the VMPFC activity and depressive symptoms.

These results reveal the brain functional basis of TER at rest, and identify a neuropsychological pathway through which TER may help ameliorate depression. Our study may provide novel neurobiological insights for early interventions targeting adolescent depression.

Sarcomas are rare mesenchymal malignancies with marked histological and molecular heterogeneity. Robust preclinical models are essential for therapeutic development, and tumor-derived cell lines remain widely used, because they are experimentally tractable and scalable. In sarcomas, the cell lines are scarce and often discontinued for distribution even after being registered in the public cell banks. Because generating and maintaining cell lines are time-consuming, labor-intensive, and costly, evidence-based prioritization of subtypes for cell line development is needed to promote sarcoma research. Here, we performed a systematic survey of sarcoma cell lines using Cellosaurus (version 54, November 2025), integrating publication status, registration in public cell banks, and current availability for distribution. From 167,127 Cellosaurus entries, we defined 87,573 unique human cell lines after excluding non-human entries and derivative or modified sublines. We identified 1080 human sarcoma cell lines, representing 1.2% of unique human cell lines. Only 230 human sarcoma cell lines were registered in public cell banks, and 139 were currently available for distribution. Most sarcoma subtypes had fewer than 10 available cell lines, and 12 sarcoma subtypes had no established cell lines. This updated landscape quantifies subtype-specific cell line scarcity and gaps in public availability and supports strategic prioritization of future sarcoma cell line development. Our findings also highlight practical measures to support long-term accessibility, including timely publication with standardized authentication reporting and guaranteeing repository redundancy via deposition in multiple public cell banks.